TRPS1 Recombinant antibody

TRPS1 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230051F10

Cat no : 83062-4-RR

Synonyms

Tricho-rhino-phalangeal syndrome type I protein, LGCR, GC79, 230051F10

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Validation Data Gallery

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  WB analysis using 83062-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83062-4-RR (TRPS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 83062-4-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 83062-4-RR (TRPS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MCF-7 using 83062-4-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TRPS1 Recombinant antibody (83062-4-RR, Clone:230051F10)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83062-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83062-4-RR against Human TRPS1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83062-4-RR targets TRPS1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRPS1 fusion protein Ag33959
• Full Name: trichorhinophalangeal syndrome I
• Calculated Molecular Weight: 1294 aa, 143 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC125020
• Gene Symbol: TRPS1
• Gene ID (NCBI): 7227
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains.