CoraLite®594-conjugated TRIM2 Monoclonal antibody

TRIM2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H9C2

Cat no : CL594-67342

Synonyms

KIAA0517, RING finger protein 86, RNF86, TRIM2, tripartite motif containing 2

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Validation Data Gallery

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  IF Staining of mouse brain using CL594-67342

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 TRIM2 antibody (CL594-67342, Clone: 1H9C2 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL594-67342

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 TRIM2 antibody (CL594-67342, Clone: 1H9C2 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67342 targets TRIM2 in IF-P applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRIM2 fusion protein Ag14637
• Full Name: tripartite motif-containing 2
• Calculated Molecular Weight: 744 aa, 82 kDa
• Observed Molecular Weight: 70-85 kDa
• GenBank Accession Number: BC011052
• Gene Symbol: TRIM2
• Gene ID (NCBI): 23321
• RRID: AB_2920118
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Tripartite motif-containing 2(TRIM2) is an E3 ubiquitin ligase which directs proteasome-mediated degradation of target proteins by the ubiquitination of NEFL and phosphorylation of BCL2L11. This protein contains an N-terminal RING domain, followed by a B-box-2 domain, a coiled-coil region, and 6 C-terminal NHL repeats. TRIM2 involves in the apoptotic response to ischemia via directing degradation of BIM protein, thus has a role in neuroprotection.