TRIM2 Monoclonal antibody

TRIM2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1H9C2

Cat no : 67342-1-Ig

Synonyms

KIAA0517, RING finger protein 86, RNF86, TRIM2, tripartite motif containing 2

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Validation Data Gallery

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  WB analysis of rat brain using 67342-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67342-1-Ig (TRIM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 67342-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67342-1-Ig (TRIM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67342-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67342-1-Ig (TRIM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67342-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67342-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 67342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 67342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 67342-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TRIM2 antibody (67342-1-Ig, Clone: 1H9C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 67342-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TRIM2 antibody (67342-1-Ig, Clone: 1H9C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse cerebellum tissue, pig brain tissue
• Positive IHC detected in: mouse brain tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

67342-1-Ig targets TRIM2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRIM2 fusion protein Ag14637
• Full Name: tripartite motif-containing 2
• Calculated Molecular Weight: 744 aa, 82 kDa
• Observed Molecular Weight: 70-85 kDa
• GenBank Accession Number: BC011052
• Gene Symbol: TRIM2
• Gene ID (NCBI): 23321
• RRID: AB_2882600
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tripartite motif-containing 2(TRIM2) is an E3 ubiquitin ligase which directs proteasome-mediated degradation of target proteins by the ubiquitination of NEFL and phosphorylation of BCL2L11. This protein contains an N-terminal RING domain, followed by a B-box-2 domain, a coiled-coil region, and 6 C-terminal NHL repeats. TRIM2 involves in the apoptotic response to ischemia via directing degradation of BIM protein, thus has a role in neuroprotection.

Publications

Species	Application	Title
human	IF	Cancer Cell Int; TRIM2 directly deubiquitinates and stabilizes Snail1 protein, mediating proliferation and metastasis of lung adenocarcinoma.; Authors - Zhaoxian Lin
human	WB,IHC	Cancers (Basel); Tripartite Motif-Containing 2, a Glutamine Metabolism-Associated Protein, Predicts Poor Patient Outcome in Triple-Negative Breast Cancer Treated with Chemotherapy; Authors - Brendah K Masisi