Filter: AllWBIHCIF

WB analysis of rat brain using 67342-1-Ig (same clone as 67342-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 67342-1-Ig (TRIM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.
WB analysis of mouse cerebellum using 67342-1-Ig (same clone as 67342-1-PBS)
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67342-1-Ig (TRIM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 67342-1-Ig (same clone as 67342-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 67342-1-Ig (TRIM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67342-1-Ig (same clone as 67342-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67342-1-Ig (same clone as 67342-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 67342-1-Ig (same clone as 67342-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 67342-1-Ig (same clone as 67342-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 67342-1-Ig (same clone as 67342-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TRIM2 antibody (67342-1-Ig, Clone: 1H9C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 67342-1-Ig (same clone as 67342-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TRIM2 antibody (67342-1-Ig, Clone: 1H9C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.

TRIM2 Monoclonal antibody, PBS Only

TRIM2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67342-1-PBS

Clone No.1H9C2

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

KIAA0517, RING finger protein 86, RNF86, TRIM2, tripartite motif containing 2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67342-1-PBS targets TRIM2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity	Human, Mouse, Rat, Pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	TRIM2 fusion protein Ag14637 Predict reactive species
Full Name	tripartite motif-containing 2
Calculated Molecular Weight	744 aa, 82 kDa
Observed Molecular Weight	70-85 kDa
GenBank Accession Number	BC011052
Gene Symbol	TRIM2
Gene ID (NCBI)	23321
RRID	AB_2882600
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9C040
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Tripartite motif-containing 2(TRIM2) is an E3 ubiquitin ligase which directs proteasome-mediated degradation of target proteins by the ubiquitination of NEFL and phosphorylation of BCL2L11. This protein contains an N-terminal RING domain, followed by a B-box-2 domain, a coiled-coil region, and 6 C-terminal NHL repeats. TRIM2 involves in the apoptotic response to ischemia via directing degradation of BIM protein, thus has a role in neuroprotection.