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CoraLite® Plus 647-conjugated TRAP1 Polyclonal antibody

TRAP1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10325

Synonyms

TNFR associated protein 1, HSPC5, HSP90L, HSP75, HSP 75

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 647-conjugated TRAP1 Polyclonal ant (CL647-10325)

    IF Staining of HepG2 using CL647-10325

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 TRAP1 antibody (CL647-10325) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated TRAP1 Polyclonal ant (CL647-10325)

    FC experiment of HeLa using CL647-10325

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human TRAP1 (CL647-10325) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-10325 targets TRAP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAP1 fusion protein Ag0400
Full Name TNF receptor-associated protein 1
Calculated Molecular Weight 80 kDa
Observed Molecular Weight
GenBank Accession NumberBC000406
Gene Symbol TRAP1
Gene ID (NCBI) 10131
RRIDAB_2920212
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the HSP90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in HSP75, but not in other HSP90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 TRAP1 antibody CL647-10325Download protocol
FC protocol for CL Plus 647 TRAP1 antibody CL647-10325Download protocol
Standard Protocols
Click here to view our Standard Protocols