TRAP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 10325-1-AP

Synonyms

TNFR associated protein 1, HSPC5, HSP90L, HSP75, HSP 75

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Validation Data Gallery

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  WB analysis of HeLa using 10325-1-AP

  WB result of TRAP1 antibody (10325-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAP1 transfected HeLa cells.

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  WB analysis using 10325-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10325-1-AP (TRAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10325-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10325-1-AP (TRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10325-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10325-1-AP (TRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 10325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10325-1-AP (TRAP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10325-1-AP (TRAP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 10325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10325-1-AP (TRAP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 10325-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10325-1-AP (TRAP1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 10325-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRAP1 antibody (10325-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells, mouse heart tissue, Raji cells, HepG2 cells, K-562 cells, NIH/3T3 cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

10325-1-AP targets TRAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAP1 fusion protein Ag0400
• Full Name: TNF receptor-associated protein 1
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 66-70 kDa, 60 kDa
• GenBank Accession Number: BC000406
• Gene Symbol: TRAP1
• Gene ID (NCBI): 10131
• RRID: AB_2303284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the HSP90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in HSP75, but not in other HSP90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).

Publications

Species	Application	Title
human	WB, IF	J Cell Biol; Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination.; Authors - Yumiko Oshima
mouse	WB,IF	Glia; The LncRNA AK018453 regulates TRAP1/Smad signaling in IL-17-activated astrocytes: A potential role in EAE pathogenesis.; Authors - Qingxiu Zhang ; KD Validated
mouse	WB	J Gerontol A Biol Sci Med Sci; Effects of Heat Stress Treatment on Age-dependent Unfolded Protein Response in Different Types of Skeletal Muscle.; Authors - Yuki Tamura
human	IHC	Sci Rep; Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.; Authors - Katsutaka Mitachi
mouse	IF	Cell Signal; Downregulated fat mass and obesity-associated protein inhibits bone resorption and osteoclastogenesis by nuclear factor-kappa B inactivation; Authors - Jinpeng Zhuang
mouse	WB	IUBMB Life; Loss of high-temperature requirement protein A2 protease activity induces mitonuclear imbalance via differential regulation of mitochondrial biogenesis in sarcopenia.; Authors - Haohan Zhou