Tested Applications
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-67315 targets TRAF2 in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | TRAF2 fusion protein Ag25387 Predict reactive species |
Full Name | TNF receptor-associated factor 2 |
Calculated Molecular Weight | 56 kDa |
Observed Molecular Weight | 53 kDa |
GenBank Accession Number | BC032410 |
Gene Symbol | TRAF2 |
Gene ID (NCBI) | 7186 |
RRID | AB_3084758 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q12933 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CL594 TRAF2 antibody CL594-67315 | Download protocol |
FC protocol for CL594 TRAF2 antibody CL594-67315 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |