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CoraLite® Plus 488-conjugated TRAF2 Monoclonal antibody

TRAF2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A4D1

Cat no : CL488-67315

Synonyms

TRAP3, TRAP, TNF receptor-associated factor 2, RING-type E3 ubiquitin transferase TRAF2, EC:2.3.2.27

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated TRAF2 Monoclonal ant (CL488-67315)

    IF Staining of HeLa using CL488-67315

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 TRAF2 antibody (CL488-67315, Clone: 2A4D1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated TRAF2 Monoclonal ant (CL488-67315)

    FC experiment of HeLa using CL488-67315

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TRAF2 (CL488-67315, Clone:2A4D1) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67315 targets TRAF2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TRAF2 fusion protein Ag25387
Full Name TNF receptor-associated factor 2
Calculated Molecular Weight 56 kDa
GenBank Accession NumberBC032410
Gene Symbol TRAF2
Gene ID (NCBI) 7186
RRIDAB_2919450
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TRAF2 antibody CL488-67315Download protocol
FC protocol for CL Plus 488 TRAF2 antibody CL488-67315Download protocol
Standard Protocols
Click here to view our Standard Protocols