CoraLite®594-conjugated TPPII Monoclonal antibody

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  IF Staining of HeLa using CL594-66017

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66017 (TPPII antibody) at dilution of 1:100.

• Featured Product
• KD/KO Validated

TPPII Monoclonal Antibody for IF/ICC

Cat No. CL594-66017

Clone No.5H6F11

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Synonyms

FLJ40359, TPP 2, TPP II, TPP2, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66017 targets TPPII in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5296

  Product name: Recombinant human TPP2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 900-1249 aa of BC039905

  Sequence: LERLKDLPFIVSHRLSNTLSLDIHENHSFALLGKKKSSNLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGSLTLSKTELGKKADVIPVHYYLIPPPTKTKNGSKDKEKDSEKEKDLKEEFTEALRDLKIQWMTKLDSSDIYNELKETYPNYLPLYVARLHQLDAEKERMKRLNEIVDAANAVISHIDQTALAVYIAMKTDPRPDAATIKNDMDKQKSTLVDALCRKGCALADHLLHTQAQDGAISTDAEGKEEEGESPLDSLAETFWETTKWTDLFDNKVLTFAYKHALVNKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTENWLPIMYPPDYCVF

• Full Name: tripeptidyl peptidase II
• Calculated Molecular Weight: 1249 aa, 138 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC039905
• Gene Symbol: TPPII
• Gene ID (NCBI): 7174
• RRID: AB_2883479
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29144
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).