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TPPII Monoclonal antibody
TPPII Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
5H6F11
Cat no : 66017-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Hela cells, Jurkat |
Positive IHC detected in | human testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells, MCF-7 cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
Product Information
The immunogen of 66017-1-Ig is TPPII Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | TPPII fusion protein Ag5296 |
Full Name | tripeptidyl peptidase II |
Calculated molecular weight | 1249 aa, 138 kDa |
Observed molecular weight | 135 kDa |
GenBank accession number | BC039905 |
Gene symbol | TPP2 |
Gene ID (NCBI) | 7174 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).
Protocols
Product Specific Protocols | |
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WB protocol for TPPII antibody 66017-1-Ig | Download protocol |
IHC protocol for TPPII antibody 66017-1-Ig | Download protocol |
IF protocol for TPPII antibody 66017-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expression. | ||
J Immunol Different Antigen-Processing Activities in Dendritic Cells, Macrophages, and Monocytes Lead to Uneven Production of HIV Epitopes and Affect CTL Recognition. |