WB analysis of HeLa using 66658-1-Ig (same clone as 66658-1-PBS)
WB result of TOMM40 antibody (66658-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOMM40 transfected HeLa cells. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
WB result of TOMM40 antibody (66658-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOMM40 transfected HeLa cells. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
WB analysis using 66658-1-Ig (same clone as 66658-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
WB analysis using 66658-1-Ig (same clone as 66658-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 66658-1-Ig (same clone as 66658-1-PBS)
pig brain tissue, rat brain tissue, and mouse brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
pig brain tissue, rat brain tissue, and mouse brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66658-1-Ig (same clone as 66658-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 66658-1-Ig (same clone as 66658-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
HepG2 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 66658-1-Ig (same clone as 66658-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
human brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 66658-1-Ig (same clone as 66658-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66658-1-Ig (TOMM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66658-1-Ig (TOMM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66658-1-Ig (same clone as 66658-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66658-1-Ig (same clone as 66658-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66658-1-Ig (same clone as 66658-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
WB analysis of HeLa using 66658-1-Ig (same clone as 66658-1-PBS)
WB result of TOMM40 antibody (66658-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOMM40 transfected HeLa cells. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
WB analysis using 66658-1-Ig (same clone as 66658-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
WB analysis using 66658-1-Ig (same clone as 66658-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 66658-1-Ig (same clone as 66658-1-PBS)
pig brain tissue, rat brain tissue, and mouse brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66658-1-Ig (same clone as 66658-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 66658-1-Ig (same clone as 66658-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 66658-1-Ig (same clone as 66658-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
IHC Figures
IHC staining of mouse liver using 66658-1-Ig (same clone as 66658-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66658-1-Ig (TOMM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of HepG2 using 66658-1-Ig (same clone as 66658-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66658-1-Ig (same clone as 66658-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66658-1-Ig (same clone as 66658-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
TOMM40 Monoclonal antibody
Catalog Number
66658-1-PBS
Citations
-
Dilutions
Applications
WB, IHC, IF/ICC, Indirect ELISA
Reactivity
human, mouse, rat, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
