Tom22 Monoclonal antibody, PBS Only

Tom22 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C2B11

Cat no : 66562-1-PBS

Synonyms

TOMM22, MSTP065, MST065, hTom22, 1C9 2

---

Validation Data Gallery

• 

  WB analysis using 66562-1-Ig (same clone as 66562-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66562-1-Ig (Tom22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66562-1-Ig (same clone as 66562-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66562-1-Ig (Tom22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (Tom22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (TOMM22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (TOMM22 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (TOMM22 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66562-1-Ig (Tom22 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66562-1-Ig (Tom22 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tom22 antibody (66562-1-Ig, Clone: 1C2B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 66562-1-Ig (same clone as 66562-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Tom22 (66562-1-Ig, Clone:1C2B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

---

Product Information

66562-1-PBS targets Tom22 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Tom22 fusion protein Ag1797
• Full Name: translocase of outer mitochondrial membrane 22 homolog (yeast)
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC009363
• Gene Symbol: TOMM22/Tom22
• Gene ID (NCBI): 56993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).