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CoraLite® Plus 488-conjugated TOM20 Recombinant antibody

TOM20 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, pig, chicken, zebrafish

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D22

Cat no : CL488-80501

Synonyms

TOMM20, TOMM 20, tom 20, MOM19, MAS20

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated TOM20 Recombinant an (CL488-80501)

    IF Staining of HeLa using CL488-80501

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TOM20 antibody (CL488-80501, Clone: 1D22 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated TOM20 Recombinant an (CL488-80501)

    FC experiment of HepG2 using CL488-80501

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TOM20 (CL488-80501, Clone:1D22) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80501 targets TOM20 in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig, chicken, zebrafish samples.

Tested Reactivity Human, mouse, rat, pig, chicken, zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TOM20 fusion protein Ag2378
Full Name translocase of outer mitochondrial membrane 20 homolog (yeast)
Calculated Molecular Weight 145 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC000882
Gene Symbol TOM20
Gene ID (NCBI) 9804
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TOM20 antibody CL488-80501Download protocol
Standard Protocols
Click here to view our Standard Protocols