Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TOM20 antibody (CL488-80501, Clone: 1D22 ) at dilution of 1:200, CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TOM20 antibody (CL488-80501, Clone: 1D22 ) at dilution of 1:200, CL594-Phalloidin (red).
FC experiment of HepG2 using CL488-80501
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TOM20 (CL488-80501, Clone:1D22) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TOM20 (CL488-80501, Clone:1D22) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions
Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Background Information
TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.
Protocols
Product Specific Protocols
IF protocol for CL Plus 488 TOM20 antibody CL488-80501
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TOM20 antibody (CL488-80501, Clone: 1D22 ) at dilution of 1:200, CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of HepG2 using CL488-80501
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TOM20 (CL488-80501, Clone:1D22) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
TOM20 Recombinant antibody
Catalog Number
CL488-80501
Citations
-
Dilutions
IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
IF/ICC, FC (Intra)
Reactivity
human, mouse, rat, pig, chicken, zebrafish
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
