TOM20 Recombinant antibody

TOM20 Recombinant Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, pig, chicken, zebrafish

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D22

Cat no : 80501-1-RR

Synonyms

Cell and organelle markers, KIAA0016, MAS20, MOM19, TOM20, TOMM20



Tested Applications

Positive WB detected inHeLa cells, zebrafish tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80501-1-RR targets TOM20 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig, chicken, zebrafish samples.

Tested Reactivity Human, mouse, rat, pig, chicken, zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TOM20 fusion protein Ag2378
Full Name translocase of outer mitochondrial membrane 20 homolog (yeast)
Calculated Molecular Weight 145 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC000882
Gene Symbol TOMM20
Gene ID (NCBI) 9804
RRIDAB_2918897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.

Protocols

Product Specific Protocols
WB protocol for TOM20 antibody 80501-1-RRDownload protocol
IHC protocol for TOM20 antibody 80501-1-RRDownload protocol
IF protocol for TOM20 antibody 80501-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols