TOM20 Recombinant antibody

TOM20 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, chicken, zebrafish

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D22

Cat no : 80501-1-RR

Synonyms

TOMM20, TOMM 20, tom 20, MOM19, MAS20

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Validation Data Gallery

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  WB analysis using 80501-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80501-1-RR (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of zebrafish using 80501-1-RR

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80501-1-RR (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 80501-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80501-1-RR (TOM20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 80501-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80501-1-RR (TOM20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 80501-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (80501-1-RR, Clone: 1D22 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 80501-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80501-1-RR against Human TOM20 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HeLa cells, zebrafish tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80501-1-RR targets TOM20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples.

• Tested Reactivity: human, mouse, rat, pig, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TOM20 fusion protein Ag2378
• Full Name: translocase of outer mitochondrial membrane 20 homolog (yeast)
• Calculated Molecular Weight: 145 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC000882
• Gene Symbol: TOM20
• Gene ID (NCBI): 9804
• RRID: AB_2918897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.