PE Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using PE-98112

  1x10^6 untreated or Con-A treated human PBMCs were surface stained with APC Anti-Human CD8 and 5 ul PE Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody (PE-98112, Clone:241344F3). Cells were not fixed.

• 

  FC experiment of human PBMCs using PE-98112

  1x10^6 Con-A treated human PBMCs were surface stained with 5 ul PE Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody (PE-98112, Clone:241344F3) (red) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9)(blue). or 1x10^6 untreated human PBMCs were surface stained with 5 ul PE Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody (PE-98112, Clone:241344F3) (black, dashed). Cells were not fixed.

TNFRSF9/CD137 Uni-rAb^TM Recombinant Antibody for FC

Cat No. PE-98112

Clone No.241344F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

TNFRSF9, ILA, CD137, 4-1BB ligand receptor, 4 1BB ligand receptor

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Tested Applications

• Positive FC detected in: Con-A treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-98112 targets TNFRSF9/CD137 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: tumor necrosis factor receptor superfamily, member 9
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: BC006196
• Gene Symbol: TNFRSF9/CD137
• Gene ID (NCBI): 3604
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q07011
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)