Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody

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  IF Staining of human tonsillitis using 98112-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TNFRSF9/CD137 antibody (98112-1-RR, Clone: 241344F3 ) at dilution of 1:180 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 98112-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TNFRSF9/CD137 antibody (98112-1-RR, Clone: 241344F3 ) at dilution of 1:180 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of human PBMCs using 98112-1-RR

  1x10^6 unstimulated or Con-A-stimulated human PBMCs were surface stained with 0.25 ug Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody (98112-1-RR, Clone: 241344F3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4). Cells were not fixed.

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  FC experiment of human PBMCs using 98112-1-RR

  1x10^6 unstimulated (black) or Con-A-stimulated human PBMCs (red) were surface stained with 0.25 ug Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody (98112-1-RR, Clone: 241344F3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), or Isotype Control (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98112-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98112-1-RR against Human TNFRSF9/CD137 were performed. The affinity constant is 10.3 nM.

TNFRSF9/CD137 {ptg:Brand} Recombinant Antibody for IF-P, FC

Cat No. 98112-1-RR

Clone No.241344F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P, FC

Synonyms

TNFRSF9, ILA, CD137, 4-1BB ligand receptor, 4 1BB ligand receptor

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue
• Positive FC detected in: Con-A-stimulated human PBMCs

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98112-1-RR targets TNFRSF9/CD137 in IF-P, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: tumor necrosis factor receptor superfamily, member 9
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: BC006196
• Gene Symbol: TNFRSF9/CD137
• Gene ID (NCBI): 3604
• RRID: AB_3672259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q07011
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)