TSG-6 Recombinant antibody, PBS Only (Capture)

TSG-6 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230438D5

Cat no : 83163-4-PBS

Synonyms

TNFAIP6, TNF alpha-induced protein 6, TNF alpha induced protein 6, Hyaluronate-binding protein, Hyaluronate binding protein

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Validation Data Gallery

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  WB analysis using 83163-4-RR (same clone as 83163-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83163-4-RR (TNFAIP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83163-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00218-3

  Sandwich ELISA standard curve of MP00218-3, Human TSG-6 Recombinant Matched Antibody Pair - PBS only. 83163-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3885. 83163-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP00218-3

  The mean TSG-6 concentration was determined to be 7.39 ng/mL in UCMSCs cell extract based on a 3.0 mg/mL extract load.

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  Sample test of MP00218-3

  Serum of five individual healthy human donors was measured. The TSG-6 concentration of detected samples was determined to be 11.38 ng/mL with a range of 0.03-52.26 ng/mL.

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  FC experiment of A549 using 83163-4-RR (same clone as 83163-4-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug TSG-6 Recombinant antibody (83163-4-RR, Clone:230438D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83163-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83163-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83163-4-RR against Human TSG-6 were performed. The affinity constant is 1.93 nM.

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Product Information

83163-4-PBS targets TSG-6 as part of a matched antibody pair:

MP00218-3: 83163-4-PBS capture and 83163-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TSG-6 fusion protein Ag3885
• Full Name: tumor necrosis factor, alpha-induced protein 6
• Calculated Molecular Weight: 277 aa, 31 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: BC030205
• Gene Symbol: TNFAIP6
• Gene ID (NCBI): 7130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.