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TSG-6 Recombinant antibody

TSG-6 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230438D5

Cat no : 83163-4-RR

Synonyms

TNFAIP6, TNF alpha-induced protein 6, TNF alpha induced protein 6, Hyaluronate-binding protein, Hyaluronate binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSG-6 Recombinant antibody (83163-4-RR)

    WB analysis using 83163-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83163-4-RR (TNFAIP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83163-4-RR

    Affinity and Kinetic Characterization of 83163-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83163-4-RR against Human TSG-6 were performed. The affinity constant is 1.93 nM.

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83163-4-RR is TSG-6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TSG-6 fusion protein Ag3885
Full Name tumor necrosis factor, alpha-induced protein 6
Calculated Molecular Weight 277 aa, 31 kDa
Observed Molecular Weight39-45 kDa
GenBank Accession NumberBC030205
Gene Symbol TNFAIP6
Gene ID (NCBI) 7130
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TSG-6 antibody 83163-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols