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CoraLite®594-conjugated TNFAIP3 Monoclonal antibody

TNFAIP3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3A11G6

Cat no : CL594-66695

Synonyms

OTUD7C, OTU domain-containing protein 7C, EC:2.3.2.-, A20p50, A20p37

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated TNFAIP3 Monoclonal antibod (CL594-66695)

    IF Staining of HeLa using CL594-66695

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated TNFAIP3 antibody (CL594-66695, Clone: 3A11G6) at dilution of 1:200, F-actin was stained using CL488-phalloidin (green) and DNA was stained by DAPI (blue).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated TNFAIP3 Monoclonal antibod (CL594-66695)

    FC experiment of HeLa using CL594-66695

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TNFAIP3 (CL594-66695, Clone:3A11G6) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66695 targets TNFAIP3 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TNFAIP3 fusion protein Ag18150
Full Name tumor necrosis factor, alpha-induced protein 3
Calculated Molecular Weight 790 aa, 90 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC114480
Gene Symbol TNFAIP3
Gene ID (NCBI) 7128
RRIDAB_2883615
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and is involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity.

Protocols

Product Specific Protocols
IF protocol for CL594 TNFAIP3 antibody CL594-66695Download protocol
FC protocol for CL594 TNFAIP3 antibody CL594-66695Download protocol
Standard Protocols
Click here to view our Standard Protocols