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CoraLite® Plus 488-conjugated TNF Alpha Monoclonal antibody

TNF Alpha Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

11H8A5

Cat no : CL488-69002

Synonyms

Cachectin, DIF, NeutraKine® TNF Alpha, TNF, TNF a, TNF alpha, TNFA, TNF-alpha, TNFSF2, Tumor necrosis factor

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488-conjugated TNF Alpha Monoclonal (CL488-69002)

    FC experiment of human PBMCs using CL488-69002

    1X10^6 PMA+ionomycin-stimulated and Monensin Solution treated human PBMCs were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human TNF Alpha (CL488-69002, Clone:11H8A5), and 0.25 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10), and 0.25 ug CoraLite® Plus 647 Anti-Human CD3 (UCHT1) (CL647-65151, Clone: UCHT1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488-conjugated TNF Alpha Monoclonal (CL488-69002)

    FC experiment of human PBMCs using CL488-69002

    1X10^6 human PBMCs were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human TNF Alpha (CL488-69002, Clone:11H8A5), and 0.25 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10), and 0.25 ug CoraLite® Plus 647 Anti-Human CD3 (UCHT1) (CL647-65151, Clone: UCHT1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of THP-1 cells using CoraLite® Plus 488-conjugated TNF Alpha Monoclonal (CL488-69002)

    FC experiment of THP-1 using CL488-69002

    1X10^6 PMA, LPS, and Brefeldin A treated THP-1 cells were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human TNF Alpha (CL488-69002, Clone:11H8A5) (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

  • Flow cytometry (FC) experiment of NK92 using CoraLite® Plus 488-conjugated TNF Alpha Monoclonal (CL488-69002)

    FC experiment of NK92 using CL488-69002

    1X10^6 PMA, ionomycin, and Brefeldin ANK92 were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TNF Alpha (CL488-69002, Clone:11H8A5) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inPMA+ionomycin-stimulated and Monensin Solution human PBMCs, PMA, LPS and Brefeldin A treated THP-1 cells, NK92

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-69002 targets TNF Alpha in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TNF Alpha fusion protein HZ-1014
Full Name tumor necrosis factor (TNF superfamily, member 2)
Gene Symbol TNF
Gene ID (NCBI) 7124
RRIDAB_2919564
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 TNF Alpha antibody CL488-69002Download protocol
Standard Protocols
Click here to view our Standard Protocols