HumanKine® recombinant human TNF alpha protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.002-0.026 ng/mL

Specific Activity

minimally 7.00 x 10⁶ IU/mg

Species Reactivity

human,mouse,rat

Purity

>95%

Publications

52

Reviews (2)

Cat no : HZ-1014

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

---

Validation Data Gallery

• 

  Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

• 

  Recombinant human TNF alpha (HZ-1014) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL.

• 

  Proteintech TNF alpha (HZ-1014) demonstrates equivalent induction cytotoxicity and EC50 to leading competitors. TNF alpha demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL.

---

Technical Specifications

• GeneID: 7124
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.002-0.026 ng/mL
• Specific Activity: minimally 7.00 x 10^6 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P01375
• Molecular Mass: 17-20 kDa reduced and non-reduced, non-disulfide bonded homotrimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse,rat

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Whitepapers

Time-Saving Dendritic Cell Generation No Medium Change - Less Reagents With HumanKine® GM-CSF and HumanKine IL4

Humankine® TNF Alpha: Authentic Trimer – High Efficiency, Human Cell Expressed

Background

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane-bound protein and free soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced mostly by activated macrophages, CD4+ lymphocytes, NK cells, neutrophils, eosinophils, and neurons. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation (PMID: 20194223).

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

Publications

Species	Title
human	Nat Cell Biol; A20 promotes metastasis of aggressive basal-like breast cancers through multi-monoubiquitylation of Snail1.; Authors - Ji-Hyung Lee
mouse	ACS Nano; Coassembly Nanomedicine Mediated by Intermolecular Interactions Between Methotrexate and Baricitinib for Improved Rheumatoid Arthritis Treatment; Authors - Hehe Xiong
human	Acta Pharm Sin B; Intravenous route to choroidal neovascularization by macrophage-disguised nanocarriers for mTOR modulation.; Authors - Weiyi Xia
human	Hepatology; Toll-like receptor 2-mediated innate immune response in human nonparenchymal liver cells toward adeno-associated viral vectors.; Authors - MariannaHösel
human	Cell Death Dis; LncRNA UCA1 promotes keratinocyte-driven inflammation via suppressing METTL14 and activating the HIF-1α/NF-κB axis in psoriasis; Authors - Yibo Hu
human	Biosens Bioelectron; Real-time monitoring of cell mechanical changes induced by endothelial cell activation and their subsequent binding with leukemic cell lines.; Authors - LiangTan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Ashish (Verified Customer) (10-21-2024)

Our lab uses this product in cell culture as one of the necroptosis-inducing agents.

• Applications: Cell culture
• Cell Tissue Type: HT29, BeWo

FH

Susanne (Verified Customer) (08-12-2021)

The product was used to enhance the production of ROS.

• Applications: Cell culture
• Cell Tissue Type: HEK293