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TNC/Tenascin-C Recombinant antibody, PBS Only (Capture)

TNC/Tenascin-C Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240844A2

Cat no : 83767-1-PBS

Synonyms

Tenascin, TNC, Hexabrachion, GP 150-225, GP 150 225

Validation Data Gallery

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  • Western Blot (WB) analysis of U-87 MG cells using TNC/Tenascin-C Recombinant antibody (83767-1-RR)

    WB analysis of U-87 MG using 83767-1-RR (same clone as 83767-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 83767-1-RR (TNC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83767-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00755-3

    Cytometric bead array standard curve of MP00755-3

    Cytometric bead array standard curve of MP00755-3, TNC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83767-1-PBS. Detection antibody: 83767-4-PBS. Standard: Ag27122. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83767-1-RR

    Affinity and Kinetic Characterization of 83767-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83767-1-RR against Human TNC/Tenascin-C were performed. The affinity constant is below 1 pM.

Product Information

83767-1-PBS targets TNC/Tenascin-C as part of a matched antibody pair:

MP00755-3: 83767-1-PBS capture and 83767-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TNC/Tenascin-C fusion protein Ag27122
Full Name tenascin C
Calculated Molecular Weight 2201 aa, 241 kDa
Observed Molecular Weight220-350 kDa, 190-240 kDa
GenBank Accession NumberBC151843
Gene Symbol Tenascin C
Gene ID (NCBI) 3371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.