TNC/Tenascin-C Recombinant antibody

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  WB analysis of U-87 MG using 83767-1-RR

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 83767-1-RR (TNC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 83767-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83767-1-RR (TNC/Tenascin-C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 83767-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83767-1-RR (TNC/Tenascin-C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83767-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83767-1-RR against Human TNC/Tenascin-C were performed. The affinity constant is below 1 pM.

TNC/Tenascin-C Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 83767-1-RR

Clone No.240844A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Tenascin, TNC, Hexabrachion, GP 150-225, GP 150 225

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Tested Applications

• Positive WB detected in: U-87 MG cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83767-1-RR targets TNC/Tenascin-C in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TNC/Tenascin-C fusion protein Ag27122
• Full Name: tenascin C
• Calculated Molecular Weight: 2201 aa, 241 kDa
• Observed Molecular Weight: 220-350 kDa, 190-240 kDa
• GenBank Accession Number: BC151843
• Gene Symbol: Tenascin C
• Gene ID (NCBI): 3371
• RRID: AB_3671361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P24821
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.