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TNC/Tenascin-C Recombinant antibody

TNC/Tenascin-C Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240844A2

Cat no : 83767-1-RR

Synonyms

Tenascin, TNC, Hexabrachion, GP 150-225, GP 150 225

Validation Data Gallery

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  • Western Blot (WB) analysis of U-87 MG cells using TNC/Tenascin-C Recombinant antibody (83767-1-RR)

    WB analysis of U-87 MG using 83767-1-RR

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 83767-1-RR (TNC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83767-1-RR

    Affinity and Kinetic Characterization of 83767-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83767-1-RR against Human TNC/Tenascin-C were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inU-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83767-1-RR is TNC/Tenascin-C Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TNC/Tenascin-C fusion protein Ag27122
Full Name tenascin C
Calculated Molecular Weight 2201 aa, 241 kDa
Observed Molecular Weight220-350 kDa, 190-240 kDa
GenBank Accession NumberBC151843
Gene Symbol Tenascin C
Gene ID (NCBI) 3371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.

Protocols

Product Specific Protocols
WB protocol for TNC/Tenascin-C antibody 83767-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols