TMEM175 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TMEM175 Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1E7E1

Cat no : 68172-1-PBS

Synonyms

Transmembrane protein 175, hTMEM175, 1E7E1

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Validation Data Gallery

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  WB analysis of HepG2 using 68172-1-Ig (same clone as 68172-1-PBS)

  WB result of TMEM175 antibody (68172-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM175 transfected HepG2 cells. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

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  WB analysis using 68172-1-Ig (same clone as 68172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68172-1-Ig (same clone as 68172-1-PBS)

  Mouse brain was subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 68172-1-Ig (same clone as 68172-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68172-1-Ig (same clone as 68172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 68172-1-Ig (same clone as 68172-1-PBS)

  IP result of anti-TMEM175 (IP:68172-1-Ig, 4ug; Detection:68172-1-Ig 1:4000) with mouse brain tissue lysate 1840 ug. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

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Product Information

68172-1-PBS targets TMEM175 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TMEM175 fusion protein Ag13890
• Full Name: transmembrane protein 175
• Calculated Molecular Weight: 504 aa, 56 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC005158
• Gene Symbol: TMEM175
• Gene ID (NCBI): 84286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability.