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TMEM175 Monoclonal antibody
TMEM175 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig, rabbit
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1E7E1
Cat no : 68172-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, pig liver tissue, rabbit liver tissue, HepG2 cells, mouse brain tissue, rat liver tissue, HeLa cells, HEK-293 cells, Jurkat cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68172-1-Ig targets TMEM175 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
Tested Reactivity | human, mouse, rat, pig, rabbit |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | TMEM175 fusion protein Ag13890 |
Full Name | transmembrane protein 175 |
Calculated Molecular Weight | 504 aa, 56 kDa |
Observed Molecular Weight | 55-60 kDa |
GenBank Accession Number | BC005158 |
Gene Symbol | TMEM175 |
Gene ID (NCBI) | 84286 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability.
Protocols
Product Specific Protocols | |
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WB protocol for TMEM175 antibody 68172-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |