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TMEM175 Monoclonal antibody

TMEM175 Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1E7E1

Cat no : 68172-1-Ig

Synonyms

Transmembrane protein 175, hTMEM175, 1E7E1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TMEM175 Monoclonal antibody (68172-1-Ig)

    WB analysis of HepG2 using 68172-1-Ig

    WB result of TMEM175 antibody (68172-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM175 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using TMEM175 Monoclonal antibody (68172-1-Ig)

    WB analysis using 68172-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using TMEM175 Monoclonal antibody (68172-1-Ig)

    WB analysis using 68172-1-Ig

    Mouse brain was subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using TMEM175 Monoclonal antibody (68172-1-Ig)

    WB analysis of pig liver using 68172-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TMEM175 Monoclonal antibody (68172-1-Ig)

    WB analysis using 68172-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using TMEM175 Monoclonal antibody (68172-1-Ig)

    IP experiment of mouse brain using 68172-1-Ig

    IP result of anti-TMEM175 (IP:68172-1-Ig, 4ug; Detection:68172-1-Ig 1:4000) with mouse brain tissue lysate 1840 ug.

Tested Applications

Positive WB detected inLNCaP cells, pig liver tissue, rabbit liver tissue, HepG2 cells, mouse brain tissue, rat liver tissue, HeLa cells, HEK-293 cells, Jurkat cells
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68172-1-Ig targets TMEM175 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMEM175 fusion protein Ag13890
Full Name transmembrane protein 175
Calculated Molecular Weight 504 aa, 56 kDa
Observed Molecular Weight55-60 kDa
GenBank Accession NumberBC005158
Gene Symbol TMEM175
Gene ID (NCBI) 84286
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability.

Protocols

Product Specific Protocols
WB protocol for TMEM175 antibody 68172-1-IgDownload protocol
IP protocol for TMEM175 antibody 68172-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols