CoraLite®594-conjugated TMEM173/STING Polyclonal antibody

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• KD/KO Validated

TMEM173/STING Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Publications

2

Cat no : CL594-19851

Synonyms

STING, NET23, MPYS, MITA, ERIS

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-19851

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-19851

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

• 

  IF Staining of HT-29 using CL594-19851

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

• 

  IF Staining of HepG2 using CL594-19851

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

• 

  FC experiment of THP-1 using CL594-19851

  1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human TMEM173/STING (CL594-19851) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue
• Positive IF/ICC detected in: HT-29 cells, human tonsillitis tissue, HepG2 cells
• Positive FC (Intra) detected in: THP-1 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• FC: See 1 publications below

Product Information

CL594-19851 targets TMEM173/STING in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM173/STING fusion protein Ag13921
• Full Name: transmembrane protein 173
• Calculated Molecular Weight: 379 aa, 42 kDa
• Observed Molecular Weight: 35-40 kDa, 80 kDa
• GenBank Accession Number: BC047779
• Gene Symbol: STING
• Gene ID (NCBI): 340061
• RRID: AB_2919854
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.

Publications

Species	Application	Title
mouse	IF	J Neuroinflammation; The BET PROTAC inhibitor dBET6 protects against retinal degeneration and inhibits the cGAS-STING in response to light damage; Authors - Xingfei Zhu
mouse	FC	Mol Pharm; STING-Targeted PET Imaging for Specific Detection and Therapeutic Monitoring of Myocarditis; Authors - Zhou Ye

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C