• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19851

Synonyms

FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, TMEM173/STING, transmembrane protein 173

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CoraLite® Plus 488-conjugated TMEM173/STING Polycl (CL488-19851)

    IF Staining of HT-29 using CL488-19851

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CoraLite® Plus 488 TMEM173/STING antibody (CL488-19851) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated TMEM173/STING Polycl (CL488-19851)

    IF Staining of HepG2 using CL488-19851

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated TMEM173 antibody (CL488-19851) at dilution of 1:400.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated TMEM173/STING Polycl (CL488-19851)

    FC experiment of HepG2 using CL488-19851

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TMEM173/STING (CL488-19851) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHT-29 cells, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-19851 is TMEM173/STING Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM173/STING fusion protein Ag13921
Full Name transmembrane protein 173
Calculated molecular weight 379 aa, 42 kDa
GenBank accession numberBC047779
Gene symbol TMEM173
Gene ID (NCBI) 340061
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TMEM173/STING antibody CL488-19851Download protocol
FC protocol for CL Plus 488 TMEM173/STING antibody CL488-19851Download protocol
Standard Protocols
Click here to view our Standard Protocols