TMEM173/STING Polyclonal antibody

• Featured Product
• KD/KO Validated

TMEM173/STING Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, Blocking assay

Conjugate

Unconjugated

Publications

333

Reviews (1)

Cat no : 19851-1-AP

Synonyms

STING, NET23, MPYS, MITA, ERIS

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Validation Data Gallery

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  WB analysis of THP-1 using 19851-1-AP

  WB result of TMEM173/STING antibody (19851-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells.

• 

  WB analysis of HL-60 using 19851-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19851-1-AP

  WB result of TMEM173/STING antibody (19851-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and TMEM173/STING knockout HepG2 cells.

• 

  WB analysis using 19851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 19851-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 19851-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19851-1-AP

  WB result of TMEM173/STING antibody (19851-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected HEK-293 cells.

• 

  WB analysis using 19851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 19851-1-AP

  IP result of anti-TMEM173/STING (IP:19851-1-AP, 4ug; Detection:19851-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.

• 

  IHC staining of human tonsillitis using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 19851-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 19851-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HT-29 using 19851-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of THP-1 using 19851-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TMEM173/STING antibody (19851-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of THP-1 using 19851-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TMEM173/STING (19851-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HL-60 cells, HEK-293 cells, HepG2 cells, mouse kidney tissue, mouse spleen tissue, Raji cells, THP-1 cells, HT-29 cells, mouse thymus tissue, C2C12 cells, RAW 264.7 cells, mouse testis tissue
• Positive IP detected in: mouse spleen tissue
• Positive IHC detected in: human tonsillitis tissue, rat thymus tissue, mouse thymus tissue, human spleen tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue
• Positive IF/ICC detected in: HT-29 cells, human tonsillitis tissue, THP-1 cells
• Positive FC (Intra) detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 45 publications below
• WB: See 276 publications below
• IHC: See 54 publications below
• IF: See 103 publications below
• IP: See 12 publications below
• FC: See 3 publications below
• CoIP: See 7 publications below

Product Information

19851-1-AP targets TMEM173/STING in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, bovine, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM173/STING fusion protein Ag13921
• Full Name: transmembrane protein 173
• Calculated Molecular Weight: 379 aa, 42 kDa
• Observed Molecular Weight: 35-40 kDa, 80 kDa
• GenBank Accession Number: BC047779
• Gene Symbol: STING
• Gene ID (NCBI): 340061
• RRID: AB_10665370
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).

Publications

Species	Application	Title
mouse	IF	Nature; Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C.; Authors - Ting-Ting Chu ; KO Validated
mouse	WB	Nature; eccDNAs are apoptotic products with high innate immunostimulatory activity; Authors - Yuangao Wang ; KO Validated
human	WB	Science; The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling.; Authors - Mena Abdel-Nour
human	WB,IF	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng ; KD Validated
human	WB	Cancer Cell; Mutant p53 suppresses innate immune signaling to promote tumorigenesis.; Authors - Monisankar Ghosh ; KD Validated
mouse	IF	Nat Immunol; Homeostatic regulation of STING protein at the resting state by stabilizer TOLLIP.; Authors - Vladislav Pokatayev

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Will (Verified Customer) (04-25-2019)

good antibody, will order again

• Applications: Western Blot,

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