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TMEM173/STING Polyclonal antibody
TMEM173/STING Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, FC, CoIP, Blocking assay, ELISA
Conjugate
Unconjugated
210
Cat no : 19851-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HepG2 cells, mouse spleen tissue, HL-60 cells, THP-1 cells, Raji cells, HT-29 cells, mouse thymus tissue, C2C12 cells |
Positive IP detected in | mouse spleen tissue |
Positive IHC detected in | human tonsillitis tissue, rat thymus tissue, mouse thymus tissue, human spleen tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HT-29 cells, human tonsillitis tissue |
Positive FC detected in | THP-1 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
19851-1-AP targets TMEM173/STING in WB, IP, IHC, IF, FC, CoIP, Blocking assay, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TMEM173/STING fusion protein Ag13921 |
Full Name | transmembrane protein 173 |
Calculated molecular weight | 379 aa, 42 kDa |
Observed molecular weight | 35-40 kDa, 80 kDa |
GenBank accession number | BC047779 |
Gene symbol | TMEM173 |
Gene ID (NCBI) | 340061 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).
Protocols
Product Specific Protocols | |
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WB protocol for TMEM173/STING antibody 19851-1-AP | Download protocol |
IHC protocol for TMEM173/STING antibody 19851-1-AP | Download protocol |
IF protocol for TMEM173/STING antibody 19851-1-AP | Download protocol |
IP protocol for TMEM173/STING antibody 19851-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C.
| ||
Nature eccDNAs are apoptotic products with high innate immunostimulatory activity
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Science The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling. | ||
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion
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Cancer Cell Mutant p53 suppresses innate immune signaling to promote tumorigenesis.
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Nat Immunol Homeostatic regulation of STING protein at the resting state by stabilizer TOLLIP. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Will (Verified Customer) (04-25-2019) | good antibody, will order again
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