TMEM126A Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83440-2-RR (same clone as 83440-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83440-2-RR (TMEM126A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83440-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83440-2-RR (same clone as 83440-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM126A antibody (83440-2-RR, Clone: 240425A11 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83440-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83440-2-RR (same clone as 83440-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TMEM126A Recombinant antibody (83440-2-RR, Clone:240425A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83440-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00449-1

  Cytometric bead array standard curve of MP00449-1, TMEM126A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83440-2-PBS. Detection antibody: 83440-1-PBS. Standard: Ag14705. Range: 6.25-100 ng/mL.

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  Affinity and Kinetic Characterization of 83440-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83440-2-RR against Human TMEM126A were performed. The affinity constant is 0.589 nM.

TMEM126A Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 83440-2-PBS

Clone No.240425A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

TMEM126, TMEM, OPA7, 240425A11

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Product Information

83440-2-PBS targets TMEM126A as part of a matched antibody pair:

MP00449-1: 83440-2-PBS capture and 83440-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TMEM126A fusion protein Ag14705
• Full Name: transmembrane protein 126A
• Calculated Molecular Weight: 195 aa, 22 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC007875
• Gene Symbol: TMEM126A
• Gene ID (NCBI): 84233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H061
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TMEM126A is a mitochondrial inner membrane protein that is enriched in the cristae. TMEM126A is necessary for Complex I assembly or stability.Complex I (CI) is the largest enzyme of the mitochondrial respiratory chain, and its defects are the main cause of mitochondrial disease.