Talin-1 Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

Talin-1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240294G5

Cat no : 82856-18-PBS

Synonyms

TLN, talin1, talin 1, KIAA1027, ILWEQ

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Validation Data Gallery

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  WB analysis of HeLa using 82856-18-RR (same clone as 82856-18-PBS)

  WB result of Talin-1 antibody (82856-18-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Talin-1 transfected HeLa cells. This data was developed using the same antibody clone with 82856-18-PBS in a different storage buffer formulation.

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  WB analysis using 82856-18-RR (same clone as 82856-18-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82856-18-RR (Talin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82856-18-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82856-18-RR (same clone as 82856-18-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82856-18-RR (Talin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-18-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82856-18-RR (same clone as 82856-18-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82856-18-RR (Talin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-18-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 82856-18-RR (same clone as 82856-18-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82856-18-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 82856-18-RR (same clone as 82856-18-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82856-18-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 82856-18-RR (same clone as 82856-18-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 82856-18-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82856-18-RR (same clone as 82856-18-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Tln1 Recombinant Antibody (82856-18-RR, Clone:240294G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82856-18-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 82856-18-RR (same clone as 82856-18-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Talin-1 Recombinant Antibody (82856-18-RR, Clone: 240294G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82856-18-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82856-18-RR

  Biolayer interferometry (BLl) kinetic assays of 82856-18-RR against Human TLN1 were performed. The affinity constant is 0.923 nM.

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Product Information

The immunogen of 82856-18-PBS is Talin-1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Talin-1 fusion protein Ag5367
• Full Name: talin 1
• Calculated Molecular Weight: 2541 aa, 270 kDa
• Observed Molecular Weight: ~270 kDa
• GenBank Accession Number: BC042923
• Gene Symbol: Talin-1
• Gene ID (NCBI): 7094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).