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Talin-1 Recombinant antibody
Talin-1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240294G5
Cat no : 82856-18-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-12 cells, HeLa cells, C6 cells, MCF-7 cells, HUVEC cells, NIH/3T3 cells, HepG2 cells |
Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
Positive FC (Intra) detected in | A549 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 82856-18-RR is Talin-1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Talin-1 fusion protein Ag5367 |
Full Name | talin 1 |
Calculated Molecular Weight | 2541 aa, 270 kDa |
Observed Molecular Weight | ~270 kDa |
GenBank Accession Number | BC042923 |
Gene Symbol | Talin-1 |
Gene ID (NCBI) | 7094 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).
Protocols
Product Specific Protocols | |
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WB protocol for Talin-1 antibody 82856-18-RR | Download protocol |
IHC protocol for Talin-1 antibody 82856-18-RR | Download protocol |
IF protocol for Talin-1 antibody 82856-18-RR | Download protocol |
FC protocol for Talin-1 antibody 82856-18-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |