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Talin-1 Recombinant antibody

Talin-1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240294G5

Cat no : 82856-18-RR

Synonyms

TLN, talin1, talin 1, KIAA1027, ILWEQ



Tested Applications

Positive WB detected inPC-12 cells, HeLa cells, C6 cells, MCF-7 cells, HUVEC cells, NIH/3T3 cells, HepG2 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82856-18-RR is Talin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Talin-1 fusion protein Ag5367
Full Name talin 1
Calculated Molecular Weight 2541 aa, 270 kDa
Observed Molecular Weight~270 kDa
GenBank Accession NumberBC042923
Gene Symbol Talin-1
Gene ID (NCBI) 7094
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

Protocols

Product Specific Protocols
WB protocol for Talin-1 antibody 82856-18-RRDownload protocol
IHC protocol for Talin-1 antibody 82856-18-RRDownload protocol
IF protocol for Talin-1 antibody 82856-18-RRDownload protocol
FC protocol for Talin-1 antibody 82856-18-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols