WB result of Talin-1 antibody (82856-18-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Talin-1 transfected HeLa cells.
WB result of Talin-1 antibody (82856-18-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Talin-1 transfected HeLa cells.
WB analysis using 82856-18-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82856-18-RR (Talin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82856-18-RR (Talin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human intrahepatic cholangiocarcinoma using 82856-18-RR
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82856-18-RR (Talin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82856-18-RR (Talin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 82856-18-RR
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82856-18-RR (Talin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82856-18-RR (Talin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 82856-18-RR
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), (CL594-Phalloidin, red).
FC experiment of HeLa using 82856-18-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Tln1 Recombinant Antibody (82856-18-RR, Clone:240294G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Tln1 Recombinant Antibody (82856-18-RR, Clone:240294G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of A549 using 82856-18-RR
1x10^6 A549 cells were intracellularly stained with 0.25 ug Talin-1 Recombinant Antibody (82856-18-RR, Clone: 240294G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 A549 cells were intracellularly stained with 0.25 ug Talin-1 Recombinant Antibody (82856-18-RR, Clone: 240294G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 82856-18-RR
Biolayer interferometry (BLl) kinetic assays of 82856-18-RR against Human TLN1 were performed. The affinity constant is 0.923 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A549 cells
Positive FC (Intra) detected in
A549 cells, HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
82856-18-RR targets Talin-1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).
WB result of Talin-1 antibody (82856-18-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Talin-1 transfected HeLa cells.
WB analysis using 82856-18-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82856-18-RR (Talin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human intrahepatic cholangiocarcinoma using 82856-18-RR
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82856-18-RR (Talin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 82856-18-RR
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82856-18-RR (Talin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of A549 using 82856-18-RR
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of A549 using 82856-18-RR
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of A549 using 82856-18-RR
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), (CL594-Phalloidin, red).
FC (INTRA) Figures
FC experiment of HeLa using 82856-18-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Tln1 Recombinant Antibody (82856-18-RR, Clone:240294G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of A549 using 82856-18-RR
1x10^6 A549 cells were intracellularly stained with 0.25 ug Talin-1 Recombinant Antibody (82856-18-RR, Clone: 240294G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 82856-18-RR
Biolayer interferometry (BLl) kinetic assays of 82856-18-RR against Human TLN1 were performed. The affinity constant is 0.923 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
Talin-1 Recombinant antibody
Catalog Number
82856-18-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:200-1:800 IF/ICC : 1:200-1:800 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
