CoraLite® Plus 488-conjugated TIPE2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TIPE2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15940

Synonyms

TIPE2, TNF alpha-induced protein 8-like protein 2, TNFAIP8 like protein 2, TNFAIP8L2, TNFAIP8-like protein 2, Tumor necrosis factor alpha-induced protein 8-like protein 2, Inflammation factor protein 20

---

Validation Data Gallery

• 

  IF Staining of RAW 264.7 using CL488-15940

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite® Plus 488 TIPE2 antibody (CL488-15940) at dilution of 1:200.

• 

  FC experiment of THP-1 using CL488-15940

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TIPE2 (CL488-15940) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF/ICC detected in: RAW 264.7 cells
• Positive FC (Intra) detected in: THP-1 cells
• Positive FC detected in: THP-1 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15940 targets TIPE2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIPE2 fusion protein Ag7964
• Full Name: tumor necrosis factor, alpha-induced protein 8-like 2
• Calculated Molecular Weight: 184 aa, 21 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC063014
• Gene Symbol: TIPE2
• Gene ID (NCBI): 79626
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TIPE2 (TNF-α-inducible protein 8-like 2, or TNFAIP8L2), a negative immune regulator, is essential for maintaining immune homeostasis. TIPE2 shares considerable sequence homology with members of the tumor necrosis factor-α-inducible protein 8 (TNFAIP8) family, which are thought to regulate cellular and immune homeostasis. TIPE2 acts as a negative regulator of Toll-like receptor and T-cell receptor function and is preferentially expressed in lymphoid tissues. TIPE2 plays an essential role for a signal transduction pathway that links inflammatory immune response to specific conditions after cerebral ischemia