TIPE2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TIPE2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

37

Cat no : 15940-1-AP

Synonyms

Tumor necrosis factor alpha-induced protein 8-like protein 2, TNFAIP8-like protein 2, TNFAIP8L2, TNFAIP8 like protein 2, TNF alpha-induced protein 8-like protein 2

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Validation Data Gallery

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  WB analysis using 15940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 15940-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 15940-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 15940-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lymph using 15940-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 15940-1-AP

  IP result of anti-TIPE2 (IP:15940-1-AP, 4ug; Detection:15940-1-AP 1:2000) with mouse spleen tissue lysate 1400 ug.

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  IHC staining of human lymphoma using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 15940-1-AP (TIPE2 antibody at dilution of 1:50.

• 

  IHC staining of human ovary tumor using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 15940-1-AP (TIPE2 antibody at dilution of 1:50.

• 

  IF Staining of RAW 264.7 using 15940-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TIPE2 antibody (15940-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15940-1-AP

  Immunofluorescent analysis of HepG2 cells using 15940-1-AP (TIPE2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of THP-1 using 15940-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TIPE2 (15940-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: THP-1 cells, mouse small intestine tissue, mouse cerebellum tissue, rat lymph tissue, rat spleen tissue, RAW 264.7 cells, mouse spleen tissue
• Positive IP detected in: mouse spleen tissue
• Positive IHC detected in: human lymphoma tissue, human ovary tumor tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells, HepG2 cells
• Positive FC (Intra) detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 32 publications below
• IHC: See 12 publications below
• IF: See 11 publications below
• IP: See 2 publications below

Product Information

15940-1-AP targets TIPE2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIPE2 fusion protein Ag7964
• Full Name: tumor necrosis factor, alpha-induced protein 8-like 2
• Calculated Molecular Weight: 184 aa, 21 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC063014
• Gene Symbol: TIPE2
• Gene ID (NCBI): 79626
• RRID: AB_2204663
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIPE2 (TNF-α-inducible protein 8-like 2, or TNFAIP8L2), a negative immune regulator, is essential for maintaining immune homeostasis. TIPE2 shares considerable sequence homology with members of the tumor necrosis factor-α-inducible protein 8 (TNFAIP8) family, which are thought to regulate cellular and immune homeostasis. TIPE2 acts as a negative regulator of Toll-like receptor and T-cell receptor function and is preferentially expressed in lymphoid tissues. TIPE2 plays an essential role for a signal transduction pathway that links inflammatory immune response to specific conditions after cerebral ischemia

Publications

Species	Application	Title
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
mouse	WB,IF,IP	Nat Immunol; Direction of leukocyte polarization and migration by the phosphoinositide-transfer protein TIPE2.; Authors - Svetlana A Fayngerts ; KO Validated
mouse,human	WB	Sci Adv; TIPE2 is a checkpoint of natural killer cell maturation and antitumor immunity.; Authors - Jiacheng Bi ; KO Validated
mouse	WB	Autophagy; TNFAIP8L2/TIPE2 impairs autolysosome reformation via modulating the RAC1-MTORC1 axis.; Authors - Wen Li ; KO Validated
mouse	WB	J Exp Med; TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis.; Authors - Dehong Yan ; KO Validated
human,mouse	WB	Cancer Immunol Res; Tumor Necrosis Factor-α-Induced Protein 8-Like 2 Fosters Tumor-Associated Microbiota to Promote the Development of Colorectal Cancer.; Authors - Yunwei Lou