TINP1 Monoclonal antibody, PBS Only

TINP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E3B11

Cat no : 66374-1-PBS

Synonyms

CDK105, Hairy cell leukemia protein 1, HCL G1, HUSSY29, NSA2, TINP1, YR 29

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Validation Data Gallery

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  WB analysis of L02 using 66374-1-Ig (same clone as 66374-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66374-1-Ig (same clone as 66374-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66374-1-Ig (same clone as 66374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66374-1-Ig (same clone as 66374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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Product Information

66374-1-PBS targets TINP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TINP1 fusion protein Ag8822
• Full Name: TGF beta-inducible nuclear protein 1
• Calculated Molecular Weight: 260 aa, 30 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC005288
• Gene Symbol: TINP1
• Gene ID (NCBI): 10412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TINP1 (TGF beta inducible nuclear protein 1) was widely expressed in various cell lines. TINP1 overexpression significantly promoted tumor cell proliferation, which may be associated with the downregulation of p53 expression. Furthermore, TINP1 promoted a number of cell lines to the S phase of the cell cycle. TINP1 promotes cell proliferation and significantly reduces p53 and p21 expression.