TINP1 Monoclonal antibody
TINP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1E3B11
Cat no : 66374-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | L02 cells, HEK-293 cells, HepG2 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66374-1-Ig targets TINP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | TINP1 fusion protein Ag8822 |
Full Name | TGF beta-inducible nuclear protein 1 |
Calculated Molecular Weight | 260 aa, 30 kDa |
Observed Molecular Weight | 34 kDa |
GenBank Accession Number | BC005288 |
Gene Symbol | TINP1 |
Gene ID (NCBI) | 10412 |
RRID | AB_2881752 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TINP1 (TGF beta inducible nuclear protein 1) was widely expressed in various cell lines. TINP1 overexpression significantly promoted tumor cell proliferation, which may be associated with the downregulation of p53 expression. Furthermore, TINP1 promoted a number of cell lines to the S phase of the cell cycle. TINP1 promotes cell proliferation and significantly reduces p53 and p21 expression.
Protocols
Product Specific Protocols | |
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WB protocol for TINP1 antibody 66374-1-Ig | Download protocol |
IHC protocol for TINP1 antibody 66374-1-Ig | Download protocol |
IF protocol for TINP1 antibody 66374-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |