TIMELESS Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TIMELESS Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B6G3

Cat no : 67022-1-PBS

Synonyms

TIMELESS1, timeless homolog (Drosophila), TIM1, TIM, Protein timeless homolog

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Validation Data Gallery

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  WB analysis using 67022-1-Ig (same clone as 67022-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67022-1-Ig (same clone as 67022-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67022-1-Ig (same clone as 67022-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 67022-1-Ig (same clone as 67022-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  WB analysis of JAR using 67022-1-Ig (same clone as 67022-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67022-1-Ig (same clone as 67022-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U2OS using 67022-1-Ig (same clone as 67022-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 67022-1-Ig (same clone as 67022-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human TIMELESS (67022-1-Ig, Clone:1B6G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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Product Information

67022-1-PBS targets TIMELESS in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TIMELESS fusion protein Ag6283
• Full Name: timeless homolog (Drosophila)
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC050557
• Gene Symbol: TIMELESS
• Gene ID (NCBI): 8914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.