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TIMELESS Monoclonal antibody

TIMELESS Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B6G3

Cat no : 67022-1-Ig

Synonyms

TIMELESS1, timeless homolog (Drosophila), TIM1, TIM, Protein timeless homolog



Tested Applications

Positive WB detected inHeLa cells, U2OS cells, A549 cells, JAR cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, K-562 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67022-1-Ig targets TIMELESS in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, canine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TIMELESS fusion protein Ag6283
Full Name timeless homolog (Drosophila)
Calculated Molecular Weight 139 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC050557
Gene Symbol TIMELESS
Gene ID (NCBI) 8914
RRIDAB_2882337
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.

Protocols

Product Specific Protocols
WB protocol for TIMELESS antibody 67022-1-IgDownload protocol
FC protocol for TIMELESS antibody 67022-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,canineWB

Eur J Pharmacol

TIMELESS promotes the proliferation and migration of lung adenocarcinoma cells by activating EGFR through AMPK and SPHK1 regulation

Authors - Houqing Yin
  • KD Validated