CoraLite® Plus 750 Anti-Mouse TIGIT Rabbit Recombinant Antibody

TIGIT Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

240242G1

Cat no : CL750-98026

Synonyms

Vstm3, 240242G1

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL750-98026

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD4 and 0.25 ug CoraLite® Plus 750 Anti-Mouse TIGIT Rabbit Recombinant Antibody (CL750-98026, Clone:240242G1). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL750-98026

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 750 Anti-Mouse TIGIT Rabbit Recombinant Antibody (CL750-98026, Clone: 240242G1) (red) or Isotype Control (blue). 1x10^6 untreated mouse splenocytes were stained with 0.25 ug CoraLite® Plus 750 Anti-Mouse TIGIT Rabbit Recombinant Antibody (CL750-98026, Clone: 240242G1) (black, dash). Cells were not fixed.

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL750-98026 is TIGIT Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: T cell immunoreceptor with Ig and ITIM domains
• Calculated Molecular Weight: 26kDa
• GenBank Accession Number: NP_001139797.1
• Gene Symbol: Tigit
• Gene ID (NCBI): 100043314
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).