Anti-Mouse TIGIT Rabbit Recombinant Antibody

TIGIT Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240242G1

Cat no : 98026-1-RR

Synonyms

Vstm3, 240242G1

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using 98026-1-RR

  1x10^6 unstimulated or anti-CD3/CD28 stimulated mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.25 ug Anti-Mouse TIGIT Rabbit Recombinant Antibody (98026-1-RR, Clone:240242G1) and PE-Conjugated Goat Anti-Rabbit IgG (H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98026-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98026-1-RR against Mouse TIGIT were performed. The affinity constant is 1.08 nM.

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 stimulated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98026-1-RR is TIGIT Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: T cell immunoreceptor with Ig and ITIM domains
• Calculated Molecular Weight: 26kDa
• GenBank Accession Number: NP_001139797.1
• Gene Symbol: Tigit
• Gene ID (NCBI): 100043314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).