CoraLite®594 Anti-Human TIGIT Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL594-98023-2

  1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD45RO, and 5 ul CoraLite®594 Anti-Human TIGIT Rabbit RecAb (CL594-98023-2, Clone: 240423C7) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL594-98023-2

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human TIGIT Rabbit RecAb (CL594-98023-2, Clone: 240423C7) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were not fixed. Lymphocytes were gated.

TIGIT Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL594-98023-2

Clone No.240423C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

VSTM3, VSIG9, T-cell immunoreceptor with Ig and ITIM domains, 240423C7

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98023-2 targets TIGIT in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: T cell immunoreceptor with Ig and ITIM domains
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: NM_173799.4
• Gene Symbol: TIGIT
• Gene ID (NCBI): 201633
• RRID: AB_3673543
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q495A1-1
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).