CoraLite® Plus 488 Anti-Human TIGIT Rabbit Recombinant Antibody

TIGIT Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240423C7

Cat no : CL488-98023-2

Synonyms

VSTM3, VSIG9, V-set and transmembrane domain-containing protein 3, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains

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Validation Data Gallery

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  FC experiment of human PBMCs using CL488-98023-2

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human TIGIT Rabbit Recombinant Antibody (CL488-98023-2, Clone: 240423C7) or Isotype control. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45RO. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-98023-2 is TIGIT Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: T cell immunoreceptor with Ig and ITIM domains
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: NM_173799.4
• Gene Symbol: TIGIT
• Gene ID (NCBI): 201633
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).