THAP11 Recombinant antibody

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  WB analysis using 84373-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84373-6-RR (THAP11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84373-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THAP11 antibody (84373-6-RR, Clone: 241590E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 84373-6-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug THAP11 Recombinant antibody (84373-6-RR, Clone:241590E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84373-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84373-6-RR against Human THAP11 were performed. The affinity constant is below 1 pM.

THAP11 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 84373-6-RR

Clone No.241590E5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

THAP domain-containing protein 11, RONIN, HRIHFB2206, CTG B45d, CTG B43a

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells, NCCIT cells, HT-29 cells, C2C12 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84373-6-RR targets THAP11 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: THAP11 fusion protein Ag19342
• Full Name: THAP domain containing 11
• Calculated Molecular Weight: 314 aa, 34 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC012182
• Gene Symbol: THAP11
• Gene ID (NCBI): 57215
• RRID: AB_3671909
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96EK4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.