THAP11 Recombinant antibody, PBS Only

THAP11 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241590E5

Cat no : 84373-6-PBS

Synonyms

THAP domain-containing protein 11, RONIN, HRIHFB2206, CTG B45d, CTG B43a

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Validation Data Gallery

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  WB analysis using 84373-6-RR (same clone as 84373-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84373-6-RR (THAP11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84373-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84373-6-RR (same clone as 84373-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THAP11 antibody (84373-6-RR, Clone: 241590E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84373-6-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84373-6-RR (same clone as 84373-6-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug THAP11 Recombinant antibody (84373-6-RR, Clone:241590E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84373-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84373-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84373-6-RR against Human THAP11 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 84373-6-PBS is THAP11 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: THAP11 fusion protein Ag19342
• Full Name: THAP domain containing 11
• Calculated Molecular Weight: 314 aa, 34 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC012182
• Gene Symbol: THAP11
• Gene ID (NCBI): 57215
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.