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THAP11 Recombinant antibody

THAP11 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241590E5

Cat no : 84373-6-RR

Synonyms

THAP domain-containing protein 11, RONIN, HRIHFB2206, CTG B45d, CTG B43a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THAP11 Recombinant antibody (84373-6-RR)

    WB analysis using 84373-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84373-6-RR (THAP11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using THAP11 Recombinant antibody (84373-6-RR)

    IF Staining of HeLa using 84373-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THAP11 antibody (84373-6-RR, Clone: 241590E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using THAP11 Recombinant antibody (84373-6-RR)

    FC experiment of HepG2 using 84373-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug THAP11 Recombinant antibody (84373-6-RR, Clone:241590E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84373-6-RR

    Affinity and Kinetic Characterization of 84373-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84373-6-RR against Human THAP11 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, NCCIT cells, HT-29 cells, C2C12 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84373-6-RR is THAP11 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen THAP11 fusion protein Ag19342
Full Name THAP domain containing 11
Calculated Molecular Weight 314 aa, 34 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC012182
Gene Symbol THAP11
Gene ID (NCBI) 57215
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for THAP11 antibody 84373-6-RRDownload protocol
IF protocol for THAP11 antibody 84373-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols