Thyroglobulin Monoclonal antibody, PBS Only

Thyroglobulin Monoclonal Antibody for IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D3C5

Cat no : 60272-1-PBS

Synonyms

AITD3, TG, TGN, thyroglobulin

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Validation Data Gallery

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  IHC staining of human thyroid using 60272-1-Ig (same clone as 60272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60272-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid using 60272-1-Ig (same clone as 60272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60272-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60272-1-Ig (same clone as 60272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60272-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60272-1-Ig (same clone as 60272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60272-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60272-1-Ig (same clone as 60272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60272-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60272-1-Ig (same clone as 60272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60272-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human thyroid cancer using 60272-1-Ig (same clone as 60272-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using Thyroglobulin antibody (60272-1-Ig, Clone: 4D3C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60272-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human thyroid cancer using 60272-1-Ig (same clone as 60272-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using Thyroglobulin antibody (60272-1-Ig, Clone: 4D3C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60272-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60272-1-PBS targets Thyroglobulin in IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Thyroglobulin fusion protein Ag18070
• Full Name: thyroglobulin
• Calculated Molecular Weight: 2768 aa, 305 kDa
• GenBank Accession Number: BC140933
• Gene Symbol: TG
• Gene ID (NCBI): 7038
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis.