CoraLite® Plus 488-conjugated TFDP2 Recombinant antibody

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  IF Staining of U2OS using CL488-84408-4

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 TFDP2 antibody (CL488-84408-4, Clone: 241457A12 ) at dilution of 1:200.

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  FC experiment of A431 using CL488-84408-4

  1x10^6 A431 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Tfdp2 Recombinant Antibody (CL488-84408-4, Clone:241457A12)(red), or 0.4 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

TFDP2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra)

Cat No. CL488-84408-4

Clone No.241457A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

Transcription factor Dp-2, DP2, Dp 2, 241457A12

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Tested Applications

• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84408-4 targets TFDP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TFDP2 fusion protein Ag2054
• Full Name: transcription factor Dp-2 (E2F dimerization partner 2)
• Calculated Molecular Weight: 446 aa, 49 kDa
• GenBank Accession Number: BC021113
• Gene Symbol: TFDP2
• Gene ID (NCBI): 7029
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14188
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TFDP2, also known as E2FDP2, is a coactivator of E2F factors and involves in EGF or IFN-gamma-mediated effects by enhancing or reducing active heterodimeric complexes. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis [PMID:16360038]. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of several genes whose products are involved in cell cycle regulation or DNA replication [PMID:16721044].