CoraLite® Plus 488-conjugated TFAP4 Recombinant antibody

TFAP4 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240031D5

Cat no : CL488-83196-4

Synonyms

Transcription factor AP 4, Class C basic helix-loop-helix protein 41, bHLHc41, AP 4, Activating enhancer-binding protein 4

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Validation Data Gallery

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  IF Staining of Hela using CL488-83196-4

  Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 TFAP4 antibody (CL488-83196-4, Clone: 240031D5 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-83196-4

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated TFAP4 Recombinant antibody (CL488-83196-4, Clone:240031D5)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: Hela cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83196-4 is TFAP4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TFAP4 fusion protein Ag2633
• Full Name: transcription factor AP-4 (activating enhancer binding protein 4)
• Calculated Molecular Weight: 338 aa, 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC010576
• Gene Symbol: TFAP4
• Gene ID (NCBI): 7023
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.