TDP-43 (C-terminal) Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TDP-43 (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 12892-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of A549 cells using 12892-1-AP (same clone as 12892-1-PBS)

  A549 cells (shcontrol and shRNA of TDP43) were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP43 antibody) at dilution of 1:1000. This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12892-1-AP (same clone as 12892-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 12892-1-AP (same clone as 12892-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 12892-1-AP (TARDBP Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 12892-1-AP (same clone as 12892-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12892-1-AP (same clone as 12892-1-PBS)

  IP result of anti-TDP-43 (C-terminal) (IP:12892-1-AP, 3ug; Detection:12892-1-AP 1:1000) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 12892-1-AP (same clone as 12892-1-PBS)

  IP result of anti-TDP-43 (C-terminal) (IP:12892-1-AP, 4ug; Detection:12892-1-AP 1:1000) with mouse brain tissue lysate 3000ug. This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12892-1-AP (same clone as 12892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12892-1-AP (same clone as 12892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12892-1-AP (same clone as 12892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12892-1-AP (TDP-43 (C-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 12892-1-AP (same clone as 12892-1-PBS)

  Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse brain tissue using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 12892-1-AP (same clone as 12892-1-PBS)

  Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse brain tissue using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12892-1-AP (same clone as 12892-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12892-1-AP (same clone as 12892-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:2400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 12892-1-AP (same clone as 12892-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Neuro-2a using 12892-1-AP (same clone as 12892-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using TDP-43 (C-terminal) antibody (12892-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 12892-1-AP (same clone as 12892-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (C-terminal) (12892-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 12892-1-PBS in a different storage buffer formulation.

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Product Information

12892-1-PBS targets TDP-43 (C-terminal) in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43-45 kDa, 35 kDa
• GenBank Accession Number: BC001487
• Gene Symbol: TDP-43
• Gene ID (NCBI): 23435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)

Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)